Journal: Frontiers in Microbiology

Article Title: Exposure of Aspergillus flavus NRRL 3357 to the Environmental Toxin, 2,3,7,8-Tetrachlorinated Dibenzo- p -Dioxin, Results in a Hyper Aflatoxicogenic Phenotype: A Possible Role for Caleosin/Peroxygenase (AfPXG)

doi: 10.3389/fmicb.2019.02338

Figure Lengend Snippet: Characteristic of A. flavus TCDD-exposed phenotype. (A) Photographs of 5-day-old cultures of A. flavus on PDA-plates upon the exposure to TCDD at 10 and 50 ng L –1 that referred as to TCDD10 and TCDD50, respectively, compared with non-exposed fungus control. (B,C) Variation in fungal mycelium dry weight and spore number, respectively, as a function of the exposure to TCDD at both doses. (D) Sections of Thin Layer Chromatography (TLC) plate showing the blue-fluorescent spots under the UV light that correspond to the AFB 1 secreted by the TCDD-exposed fungi compared to control. (E) Quantitative data of AFB 1 as estimated by UV-detector HPLC. All measurements were done in triplicate and the presented data are means ± SD ( n = 3). Difference between treatments and control was significant ( ∗ P < 0.05) or very significant ( ∗∗ P < 0.01) when analyzed by t -test.

Article Snippet: AF was analyzed using a Jasco LC-2000 plus series HPLC system (Jasco, United States) with a fluorescence detector (RF-10Axl, Shimadzu) (λexc 247 nm; λem 480 nm) and a C18 column (Eclipse XDB-C18 150 × 4.6 mm, 5 μm; Agilent, United States, column temperature 35°C) as described previously ( ).

Techniques: Control, Thin Layer Chromatography